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Flow Cytometry
....We have developed
a new microfluidic device utilizing multi-wavelength
detection for high-throughput Capillary Electrophoresis
(CE) analysis. In general, different fluorescent dyes
are only excited by light sources having appropriate
wavelengths. When excited by an appropriate light source,
a dye emits specific fluorescence signals of a longer
wavelength. The experiment designs and fabricates plastic
micro-CE chips capable of performing multiple-wavelength
fluorescence detection by means of multi-mode optic
fiber pairs embedded downstream of the separation channel.
....For detection purposes,
the fluorescence signals are enhanced by positioning
micro-focusing-lens structures at the outlets of the
excitation fibers and the inlets of the detection fibers,
respectively. The proposed device is capable of detecting
multiple samples labeled with different kinds of fluorescent
dyes in the same channel in a single run. The experimental
results demonstrate that various proteins, including
BSA (Bovine Serum Albumin) and β casein, can be successfully
separated and detected by coupling two light sources
of different wavelengths to the two excitation optic
fibers.
....Furthermore, the
proposed device also provides the ability to measure
the speed of sample travel in the microchannel. The
developed multi-wavelength micro-CE chip has significant
potential for the analysis of DNA and protein samples.
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