Microfluidic Chips for
High-throughput Drug Screening
....The objective of the
proposed 3-year project is to design and fabricate a
microchip system using enabling MEMS (Micro-Electro-Mechanical-System)
technology for steroid-related drug screening and identification.
The success of the proposed project will create breakthrough-enabling
microfluidic protein chips for fast drug screening.
Based on crucial platform technologies we developed
in the phase-one of the “National Science and Technology
Program in Pharmaceuticals and Biotechnology”, we will
apply these microfluidic chips for the application of
fast drug screening.
and integrated protein chips will be proposed and integrated
for this purpose in the phase-one of the program, including
PIMM (Protein-Protein Interaction Microfluidic Module)
and PIMM (Protein Identification Microfluidic Module,
PIMM). The screening mechanisms include (1) binding
assay and (2) enzyme activity, which all will be executed
using the proposed protein chips.
and integrated chip-systems will be implemented in the
(1) Cell preparation module: cells will be cultured,
counted and collected on a miniaturized chip.
(2) Nucleus preparation module: nucleus and proteins
will be collected and extracted for subsequent purpose.
(3) All-on-chip PPIMM: on-chip protein interaction will
(4) On-chip optical detection system integrated with
microfluidic chips to make compact system feasible.
....This challenging sub-project
will be implemented in MEMS Common Laboratory in NCKU,
as well as the foundry services provided in Taiwan and
USA. The efforts of the first year will be focused on
the development of key technology and individual functional
chips. In order to provide reliable test chips for other
two sub-projects, we will refine PPIMM chips developed
in the phase one of the program. The chips will be tested
extensively in the second year and the results will
be used to improve the design of the microchips. The
functional protein chips will be used by the researchers
from other two projects. The major focus of the project
in the third year will be the integration of each chip
for fast drug screening. The microchip will be demonstrated
as a powerful tool for fast screening of steroid-related