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Name K. Y. Lien  
Grade Former Members
Group F group : Microfluidic chips for fast disease diagnosis
Telephone +886-6-2761687
Fax +886-6-2761687
E-mail kangyilien@gmail.com
Homepage  
Educations
  • B.S, Engineering Science, National Cheng Kung University (2001-2004)
  • M.S, Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University (2005-2006)
  • Ph. D., Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University (2007-2009)
Experiences
  • Editorial assistant of Curriculum & Instruction Quarterly (TSSCI) (2005.7-2007.1)
  • Oral presentation at The 2nd International Meeting on Microsensors & Microsystems (IMμ2), Tainan, Taiwan, Jan. 15-18, 2006.
  • Poster presentation at The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS2006), Tokyo, Japan, Nov. 5-9, 2006.
  • Oral presentation at The 20th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 07), Kobe, Japan, Jan. 21-25, 2007.
  • Oral presentation at The 21st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 08), Tucson, Arizona, USA, Jan. 13-17, 2008.
  • Oral presentation atThe 23rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS 10), Hong Kong, China, Jan. 24-28, 2010.
Specialities and Research Interests
  • Digital Microfluids System (EWOD)
  • Polymerase Chain Reaction (PCR)
  • Magnetic Bead in biological application
  • Micro electromagnet
  • Microfluidic control systems
  • 8051 microprocesser components
Honors
  • First prize, Student Paper Competition (Electronical and Informational Group), National Cheng Kung University, 2004.
  • Second prize, National Engineering Paper Competition (Electronical and Informational Group), 2004.
  • Excellent piece, Student Paper Competition (Energy and heat flow Group), the 30th National Conference on Theoretical and Applied Mechanics, 2005.
  • Scholarship for excellent master student, Kaohsiung Computer Association, 2006.
  • Excellent piece, the 2nd Longterm Smiling Competition, 2007.
  • Second prize, Student Research Contest of the 5th National Innovation Award, 2007.
  • Award of academic research category, the 6th National Innovation Award, 2008.
  • Bronze Medal prize, the 1st Campus Venture Competition, National Cheng Kung University, 2009.
Patents
  • A. Patents:
  • 1. “Polymerase Chain Reaction Chip,” Inventors: Gwo-Bin Lee, Kang-Yi Lien, patent filed on 2007/06/01 with a patent filing No.200726838.
  • 2. “Integrated Bio-samples Pretreatment Chip,” Inventors: Gwo-Bin Lee, Huan-Yao Lei, Kang-Yi Lien, Wan-Chi Lee, patent filed on 2007/07/16 with a patent filing No.200726838.
  • 3. “Microfluidic biomedical chip for rapid diseases detection,” Inventors: Gwo-Bin Lee,Sung-Yi Yang,Kang-Yi Lien,Huan-Yao Lei,Kuo-Jean Huang, patent filed on 2009/03/16 with a patent filing No.200912310.
  • 4. “Bio-chip for rapid nucleic acids extraction,” Inventors: Gwo-Bin Lee,Kang-Yi Lien,Chien-Ju Liu, patent filed on 2009/06/16 with a patent filing No.200925593.
  • 5. “Bio-chip for rapid DNA extraction and genetic diseases detection,” Inventors: Gwo-Bin Lee,Kang-Yi Lien,Chien-Ju Liu, filing date: 2008/01/30 with a filing number 97103556.
  • 6. “Immunoassay chip,” Inventors: Gwo-Bin Lee,Huan-Yao Lei,Yu-Fang Lee,Kang-Yi Lien, filing date: 2009/3/20 with a filing number 98109045.
  • 7. “A method and microfluidic system for the synthesis of magnetic particles,” Inventors: Gwo-Bin Lee,Chen-Yi Lee,Kang-Yi Lien, filing date: 2009/5/27 with a filing number 98117735.
  • 8. “Microfluidic chip,”Inventors: Gwo-Bin Lee,Sung-Yi Yang,Kang-Yi Lien, Chen-Hsun Weng,filing date: 2009/10/30 with a filing number 98136932.
  • 9. “Immunoassay Biochip,”Inventors: Gwo-Bin Lee,Huan-Yao Lei,Yu-Fang Lee,Kang-Yi Lien, patent file to USPTO on 2010/03/09 with a filing number 12/659,422 (USA).
  • 10. “Microfluidic chip,”Inventors: Gwo-Bin Lee, Sung-Yi Yang, Kang-Yi Lien, Chen-Hsun Weng, patent file to USPTO on 2010/06/07 with a filing number 12/795,445 (USA).
  • B. Journal Papers:
  • 1. Jr-Lung Lin, Gwo-Bin Lee, Yi-Hsien Chang and Kang-Yi Lien, “Model Description of Contact Angles in Electrowetting on Dielectric Layers,” Langmuir, 22(1), 484-489, 2006. (SCI; Impact factor= 4.097)
  • 2. Kang-Yi Lien, Wan-Chi Lee, Huan-Yao Lei, and Gwo-Bin Lee, “Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic Beads for Virus Detection,” IEEE Review on Advances of Micro, Nano, and Molecular Systems, 1, 309-310, 2006.(SCI)
  • 3. Kang-Yi Lien, Wan-Chi Lee, Huan-Yao Lei and Gwo-Bin Lee, “Integrated Reverse Transcription Polymerase Chain Reaction Systems for Virus Detection,” Biosensors and Bioelectronics, 22(8), 1739-1748, 2007. (SCI; Impact factor= 5.143)
  • 4. Kang-Yi Lien, Jr-Lung Lin, Cheng-Yu Liu , Huan-Yao Lei and Gwo-Bin Lee, “Purification and Enrichment of Virus Samples Utilizing Magnetic Beads on a Microfluidic System,” Lab on a Chip, 7(7), 868-875, 2007. (SCI; Impact factor= 6.478)
  • 5. Wan-Chi Lee, Kang-Yi Lien, Gwo-Bin Lee and Huan-Yao Lei, “An Integrated Microfluidic System Using Magnetic Beads for Virus Detection,” Diagnostic Microbiology and Infectious Disease, 60(1), 51-58, 2008. (SCI; Impact factor= 2.139)
  • 6. Kang-Yi Lien, Wang-Ying Lin, Yu-Fang Lee, Chih-Hao Wang, Huan-Yao Lei and Gwo-Bin Lee, “Microfluidic System Integrated with Sample Pretreatment Device for Fast Nucleic Acid Amplification,” IEEE/ASME Journal of Microelectromechanical Systems, 17(2), 288-301, 2008. (SCI; Impact factor= 2.226)
  • 7. Sung-Yi Yang, Kang-Yi Lien, Kao-Jean Huang, Huan-Yao Lei and Gwo-Bin Lee “Micro Flow Cytometry Utilizing a Magnetic Bead-based Immunoassay for Rapid Virus Detection,” Biosensors and Bioelectronics, 24(4), 861-868, 2008. (SCI; Impact factor= 5.143)
  • 8. Kang-Yi Lien, Chien-Ju Liu, Yi-Chien Lin, Pao-Lin Kuo and Gwo-Bin Lee “Extraction of genomic DNA and detection of single nucleotide polymorphism genotyping utilizing an integrated magnetic bead-based microfluidic platform,” Microfluidics and Nanofluidics, 6, 539–555, 2009. (SCI; Impact factor= 3.314)
  • 9. Chien-Ju Liu, Kang-Yi Lien, Ching-Yi Weng, Jyh-Wei Shin, Tsuey-Yu Chang and Gwo-Bin Lee, “Magnetic-bead-based Microfluidic System for Ribonucleic Acid Extraction and Reverse Transcription Processes,” Biomedical Microdevices, 11, 339–350, 2009. (SCI; Impact factor= 2.924)
  • 10. Tsung-Min Hsieh, Ching-Hsing Luo, Jung-Hao Wang, Jr-Lung Lin, Kang-Yi Lien and Gwo-Bin Lee, “A Two-dimensional Self-compensated Micro Thermal Cycler for One-step Reverse Transcription Polymerase Chain Reaction,” Microfluidics and Nanofluidics, 6(6), 797-809, 2009. (SCI; Impact factor= 3.314)
  • 11. Kang-Yi Lien, Szu-Hsien Lee, Tieh-Jung Tsai, Tzong-Yueh Chen and Gwo-Bin Lee, “A microfluidic-based system using reverse transcription polymerase chain reactions for rapid detection of aquaculture diseases,” Microfluidics and Nanofluidics, 7, 795-806, 2009. (SCI; Impact factor= 3.314)
  • 12. Kang-Yi Lien, Chien-Ju Liu, Pao-Lin Kuo and Gwo-Bin Lee, “Microfluidic system for rapid detection of α-thalassemia-1 deletion using saliva samples,” Analytical Chemistry, 81(11), 4502–4509, 2009. (SCI; Impact factor= 5.712)
  • 13.Yu-Fang Lee, Kang-Yi Lien, Huan-Yao Lei and Gwo-Bin Lee “An integrated microfluidic system for rapid diagnosis of dengue virus infection,” Biosensors and Bioelectronics, 25, 745-752, 2009. (SCI; Impact factor= 5.143)
  • 14. Kang-Yi Lien and Gwo-Bin Lee, “Miniaturization of molecular biological techniques for gene assay,” Analyst, Vol. 135, Issue 7, pp. 1499-1518, 2010. (SCI; Impact factor= 3.761)
  • 15. Chen-Hsun Weng, Kang-Yi Lien, Sung-Yi Yang and Gwo-Bin Lee, “A suction-type, pneumatic microfluidic device for liquid transport and mixing,” in press, Microfluidics and Nanofluidics, DOI: 10.1007/s10404-010-0669-1, 2010 (SCI; Impact factor=3.286)
  • 16. Kang-Yi Lien, Ying-Hsin Chuang, Lein-Yu Hung, Keng-Fu Hsu, Wu-Wei Lai, Chung-Liang Ho, Cheng-Yang Chou and Gwo-Bin Lee, “Rapid isolation and detection of cancer cells by utilizing integrated microfluidic systems,” in press, Lab on a Chip, 10(21), 2875-2886, 2010 (SCI; Impact factor=6.342).
  • 17. Chih-Hung Wang, Kang-Yi Lien, Ting-Yu Wang, Tzong-Yueh Chen and Gwo-Bin Lee, “An integrated microfluidic loop-mediated-isothermal-amplification system for rapid sample pre-treatment and detection of viruses,” in press, Biosensors and Bioelectronics, DOI: 10.1016/j.bios.2010.08.083, 2010 (SCI; Impact factor=5.429).
  • C. Conference Papers:
  • 1. Jr-Lung Lin, Gwo-Bin Lee, Yi-Hsien Chang and Kang-Yi Lien, “Theoretical Prediction of Saturated Contact Angles in Electrowetting on Dielectric,” The 22nd National Conference on Mechanical Engineering, Taoyuan, Taiwan, Nov. 25-26, 2005.
  • 2. Kang-Yi Lien, Wan-Chi Lee, Huan-Yao Lei, and Gwo-Bin Lee, “Integrated Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic beads for Virus Detection,” The 2nd International Meeting on Microsensors & Microsystems (IMμ2), Tainan, Taiwan, Jan. 15-18, 2006.
  • 3. Kang-Yi Lien, Wan-Chi Lee, Huan-Yao Lei, and Gwo-Bin Lee, “Integrated Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic beads for Virus Detection,” The 1st IEEE-NEMS 2006, Zhuhai, China, Jan. 18-21, 2006.
  • 4. Kang-Yi Lien, Cheng-Yu Liu, Jr-Lung Lin, Hao-Yu Tseng, Huan-Yao Lei, Huei-Huang Lee and Gwo-Bin Lee, “Microfluidic Systems Integrated with Pumping/Mixing and Separating Devices Using Magnetic Beads for Virus Detection,” The 4th Conference on Precision Machinery and Manufacturing Technology, Kenting, Taiwan, May 26-28, 2006.
  • 5. Kang-Yi Lien, Cheng-Yu Liu, Jr-Lung Lin, Hao-Yu Tseng, Wan-Chi Lee, Huan-Yao Lei and Gwo-Bin Lee, “Microfluidic Systems Integrated with Pumping/Mixing and Separation Devices for Virus Detection,” The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS 2006), Tokyo, Japan, Nov. 5-9, 2006.
  • 6. Kang-Yi Lien, Wang-Ying Lin, Chih-Hao Wang, Huan-Yao Lei and Gwo-Bin Lee, “Integrated Miniature RT-PCR Systems for Bio-sample Pretreatment and Detection,” The 10th Conference on Nano Engineering and Microsystem technology, Hsinchu, Taiwan, Nov. 30-Dec. 1, 2006.
  • 7. Kang-Yi Lien, Jr-Lung Lin, Cheng-Yu Liu, Huan-Yao Lei and Gwo-Bin Lee, “Microfluidic Systems Integrated with Pumping/Mixing and Separation Devices Utilizing Magnetic Beads for Rapid Virus Detection,” The 30th National Conference on Theoretical and Applied Mechanics, Changhwa, Taiwan, Dec. 15-16, 2006.
  • 8. Kang-Yi Lien, Wang-Ying Lin, Chih-Hao Wang, Huan-Yao Lei and Gwo-Bin Lee, “Miniature RT-PCR Systems Integrated with a Sample Pretreatment Device for Virus Detection,” The 20th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 07), Kobe, Japan, Jan. 21-25, 2007.
  • 9. Sung-Yi Yang, Kang-Yi Lien, Kao-Jean Huang, Huan-Yao Lei and Gwo-Bin Lee, “Immunoassay Utilizing Magnetic Beads for Rapid Virus detection in the Microfluidic Flow Cytometer System,” The 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS 2007), Paris, France, Oct. 7-11, 2007.
  • 10. Kang-Yi Lien, Chien-Ju Liu, Jyh-Wei Shin, Tsuey-Yu Chang and Gwo-Bin Lee, “Bead-Based Miniature Microfluidic Systems for Rapid RNA Extraction And Reverse Transcription,” ASME Micro/Nanoscale Heat Transfer International Conference (MNHT 08), Tainan, Taiwan, Jan. 6-9, 2008.
  • 11. Kang-Yi Lien, Chien-Ju Liu and Gwo-Bin Lee, “Magnetic-bead-based Microfluidic Systems for Detection of Genetic Diseases,” The 21st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 08), Tucson, Arizona, USA, Jan. 13-17, 2008.
  • 12. Sung-Yi Yang, Kang-Yi Lien, Kao-Jean Huang, Huan-Yao Lei and Gwo-Bin Lee, “Immunoassay Utilizing Magnetic Beads-based Microfluidic system for Rapid Virus detection,” The 25th National Conference on Mechanical Engineering, Changhua, Taiwan, Nov. 21-22, 2008.
  • 13. Kang-Yi Lien, Chien-Ju Liu, Pao-Lin Kuo and Gwo-Bin Lee, “Bead-based Microfluidic Platform Integrated with Optical Detection Devices for Rapid Detection of Genetic Deletion from Saliva,” The 22nd IEEE International Conference on Micro Electro Mechanical Systems (MEMS 09), Sorrento, Italy, Jan. 25-29, 2009.
  • 14. Yu-Fang Lee, Kang-Yi Lien, Huan-Yao Lei and Gwo-Bin Lee, “A Magnetic-Bead Based Microfludic System for Rapid Detection of Immunoglobulins,” The 15th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducer 09), Denver, Colorado, USA, Jun. 21-25, 2009.
  • 15. Yu-Fang Lee, Kang-Yi Lien, Huan-Yao Lei, Ruo-Chi Hsu and Gwo-Bin Lee, “An Immunomagnetic-bead-based Microfluidic System for Rapid Diagnosis of Influenza Infection,” The 5th International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, Jan. 20-23, 2010.
  • 16. Chen-Yi Lee, Song-Bin Huang, Kang-Yi Lien, Gwo-Bin Lee, Ming-Yang Lin, “Tunable Magnetic Alginate Microspheres by Using a Microfluidic Device,” The 5th International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, Jan. 20-23, 2010.
  • 17. Kang-Yi Lien, Chien-Ju Liu, Keng-Fu Hsu, Cheng-Yang Chou and Gwo-Bin Lee, “A Magnetic Bead-based 3D Microfluidic Incubator for Rapid Purification and Detection of Tumor Cells,” The 23rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS 10), Hong Kong, China, Jan. 24-28, 2010.
  • 18. Johannes Piepenbrock, Artur Scholz, Gwo-Bin Lee, Chih-Hung Wang, Kang-Yi Lien, Jyh-Ching Juang, Jiun-Jih Miau, Bernd Dachwald, “Bio-Lab - Case Study of a Biological Experiment for CubeSat Missions,” Small Satellites Systems and Services-The 4S Symposium 2010, Madeira, Portugal, May 31-June 4, 2010.
  • 19. Chih-Hung Wang, Kang-Yi Lien, Ting-Yu Wang, Tzong-Yueh Chen and Gwo-Bin Lee, “Integrated Microfluidic Loop-mediated-isothermal-amplification System for Rapid Diagnosis of Aquaculture Virus,” The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS 2010), Groningen, The Netherlands, Oct. 3-7, 2010.
  • 20. Kang-Yi Lien, Lien-Yu Hung, Huan-Yao Lei and Gwo-Bin Lee, “A Magnetic-bead-based Immunoassay for Rapid Purification and Detection of Influenza Viruses Utilizing Suction-type Microfluidic Systems,” The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS 2010), Groningen, The Netherlands, Oct. 3-7, 2010.
  • D. Book
  • 1. Kang-Yi Lien, “Miniature Microfluidic System Integrated with a Sample Pretreatment Device for Rapid Nucleic Acid Amplification,” M. S. thesis, NCKU, 2007.
  • 2. Kang-Yi Lien, Huan-Yao Lei and Gwo-Bin Lee, “Dengue detection chips,” Invited chapter of Dengue Disease (ISBN:978-81-308-0290-9), Research Signpost Publisher, India, pp. 87-104, 2008.
  • 3. Kang-Yi Lien, “Integrated microfluidic systems for the detection of cancer, infectious and genetic diseases,” PhD dissertation, National Cheng Kung University, 2010.
  • E. Other Publications
  • 1. Kang-Yi Lien, Wan-Chi Lee, Huan-Yao Lei and Gwo-Bin Lee, “Integrated Reverse Transcription Polymerase Chain Reaction Systems for Virus Detection,” BANYAN-Research Express@NCKU, Volume 1(5), 2007.
Research Subject Microfluidic systems for sample pretreatmemt and diseases detection